REDBIO México 2010

Folio: 74
Tipo de presentación: Cartel
Tema: Otro

Phenolic content and antioxidant activity of plant and calli Theobroma cacao L. extracts.

Janet Quiñones * (1), Reinaldo Trujillo (1), Troy Augustine (1), Yanelis Capdesuñer (1), Yemeys Quirós (1), Janetsy Borroto (1), Martha Hernández. (1).
* Autor para correspondencia (Corresponding author) jquinones@bioplantas.cu

RESUMEN (ABSTRACT)
"Cocoa-derived foods are rich in phenol and are obtained from the fermented, roasted and milled seeds of Theobroma cacao L. Of special interest are plant-based phenolic metabolites due to their potent antioxidant activity and wide range of pharmacologic properties including anticancer, antioxidant, and platelet aggregation inhibition activity. The antioxidant activities of phenolic compounds are mainly due to their redox properties, which can play an important role in adsorbing and neutralizing free radicals. The aim of this work were to determinate the phenolic content in plant of Theobroma cacao L., the antioxidant activity of the extracts Leaves, branches, seeds, flowers and roots of field plant, and callus of staminodes, petals and nucelus were analyzed. The nucelus, petals and staminoids were culture on a callogenesis medium and the callus grown were measured. The Phenolic content was determinated with Folin-Ciocalteu reagent according to the method of Gurr et al. (1992). The procedure for performing antioxidant activity were based on Harding and Benson (1995) and Ait Barka et al.(2000) for malondialdehyde and aldehyde levels respectably. The staminodes and petals showed a higher grown of callus than nucelus. The highest phenolic content was recorded in seeds showing significant statistical difference with other organs, the callus of nucelus showed more phenolic content than the other callus evaluated. The Branches and flowers displayed the highest antioxidant activity with the lowest malondialdehide and other aldehide value followed by old leaves, seed, young leaves and root. The callus of staminodes, petals and nucelus showed a similar antioxidant activity."