REDBIO México 2010

Folio: 599
Tipo de presentación: Oral
Tema: Mejoramiento Genético Vegetal
Este trabajo se presentará en "Presentaciones Libres 11: Biodiversidad, Mejoramiento y Marcadores Moleculares" el Miercoles dentro del horario 14:20 a 16:20

Development of functional markers and SNP-genotyping technologies for association mapping in sunflower (Helianthus annuus)

Corina M. Fusari * (1), Verónica V. Lia (1), Carolina Troglia (2), María Valeria Moreno (3), Daniel Álvarez (4), Julio Di Rienzo (5), Jeremías E. Zubrzycki (1), Verónica Nishinakamasu (1), Carla A. Maringolo (2), Andrea F. Puebla (1), H. Esteban Hopp (1), Ruth A. Heinz (1), Norma B. Paniego (1).
* Autor para correspondencia (Corresponding author) cfusari@cnia.inta.gov.ar

1- Instituto de Biotecnología, CICVyA-INTA Castelar, Buenos Aires, Argentina; 2- Unidad Integrada EEA INTA Balcarce-UNMdP, Mar del Plata, Buenos Aires, Argentina; 3- Laboratorio de Biotecnología – INTA Manfredi, Córdoba, Argentina; 4- Banco Activo de Girasol-INTA Manfredi, Córdoba, Argentina; 5- Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, Córdoba, Argentina

RESUMEN (ABSTRACT)
"The analysis of DNA sequence variation is of major importance in genetic studies. The abundance, ubiquity and interspersed nature of SNPs, together with the potential of automatic high-throughput analysis make them ideal candidates for construction of high density genetic maps, QTL fine mapping, marker assisted plant breeding and genetic association studies. For association mapping, besides the main aim of establishing the levels of nucleotide diversity and the extent of linkage disequilibrium (LD), it is important to settle simple, accurate and cost-effective SNP genotyping technologies to be useful in the characterization of hundreds of individuals or on pooled samples either for candidate gene or genome-wide approaches. Nucleotide diversity and LD were assessed upon sequence information of 31 genes and 19 sunflower inbred lines. The relatively high frequency of SNPs found (1SNP/61 pb, Tetha=0.0056), along with the predicted extent of LD over distances of 100 kbp (r2~0.1) allowed conducting an association mapping strategy through the candidate gene approach. In addition, two SNP genotyping methods for scale-up throughput were explored: (1) CEL1 endonuclease cleavage of missmatch heteroduplex and (1) denaturing high-performance liquid chromatography (dHPLC). Different alternatives for candidate gene selection for Sclerotinia head rot resistance-association mapping strategy were also investigated. Sixteen candidate genes were tested for association in a total of 94 sunflower germplasm accessions evaluated for disease response. Results from these experiments suggest that association mapping through the candidate gene approach is a powerful tool for complex trait dissection in sunflower."