REDBIO México 2010

Folio: 440
Tipo de presentación: Cartel
Tema: Biotecnología Forestal

In vitro propagation of Cedrela odorata L. from juvenile shoots

Rolando García * (1), Miladys Delgado (2), Yailín González (2), Aníbal González (2), Karla Quiroz (1), Miguel Garriga (3), Peter D. S. Caligari (3), Basilio Carrasco. (4).
* Autor para correspondencia (Corresponding author) rgarciag@ucm.cl

1- Facultad de Ciencias Agrarias y Forestales. Universidad Católica del Maule. Campus San Miguel. Avda. San Miguel Nº 3605, Casilla 617. Talca, Chile; 2- Estación Experimental Forestal de Camagüey, P.O. Box 405, C.P. 70100. Camagüey, Cuba; 3- Instituto de Biología Vegetal y Biotecnología. Universidad de Talca. 2 Norte No. 845. Talca, Chile; 4- Departamento de Fruticultura. Facultad de Agronomía e Ingeniería Forestal. Pontificia Universidad Católica de Chile. Vicuña Mackenna 4860, Casilla 306, Correo 22. Santiago de Chile, Chile

RESUMEN (ABSTRACT)
"Cedrela odorata L. is one of the most important timber species currently traded in the Caribbean and Central America; however, it has been intensively exploited. In vitro propagation tools can help to develop new plantations and to assist in the establishment of improvement programs for this species. Here we report a protocol on establish this species into in vitro conditions. Disinfection of node explants with 5% propiconazole CE 25 during three minutes resulted in 100% explant disinfection and 60% morphogenic response on those established explants. Shoot development was optimized by cultivating in vitro node explants in Murashige and Skook basal medium supplemented with 2 mg/L of 6-bencilaminopurine and 3 mg/L of naftalenacetic acid. This medium resulted in shoot development in 100% of the in vitro node explants with an average of 3.93 cm height, as average. Rooting was also stimulated six weeks after individualization of the regenerated plants on the same medium with an average of 3.9 roots per plant. In vitro plants did not show morphologic differences compared to the ex vitro seedlings."