REDBIO México 2010

Folio: 435
Tipo de presentación: Cartel
Tema: Estrés Biótico

Characterization of three metallothionein-like genes obtained from a subtractive library in ‘Calcutta 4’-Mycosphaerella fijiensis Morelet interaction

Milady F. Mendoza-Rodríguez1*, Aminael Sánchez-Rodríguez1, Orelvis Portal1, Mayra Acosta-Suárez1, Berkis Roque1, Yovanni Izquierdo1, Monica Höfte2 and Elio Jiménez1
1Instituto de Biotecnología de las Plantas. Universidad Central 'Marta Abreu' de Las Villas. Carretera a Camajuaní, km 5.5. Santa Clara, Villa Clara, Cuba. CP 54 830. e-mail: milady@ibp.co.cu 2Laboratory of Phytopathology. Department of Crop Protection. Faculty of Bioscience Engineering. University of Gent. Belgium.

RESUMEN (ABSTRACT)
"Metallothioneins (MTs) are small, Cys-rich proteins which are involved in reactive oxygen species (ROS) scavenging and metal homeostasis. In contrast to the vertebrate forms, knowledge about members of the plant metallothionein is still scarcely. In this study, three complementary deoxyribonucleic acid (cDNA) encoding type-3 MT-like genes were characterized, from an expressed sequence tag collection, obtained from a polymerase chain reaction based suppression subtractive hybridization approach from ‘Calcutta 4’ leaves inoculated with Mycosphaerella fijiensis Morelet. Sequence alignment, domains findings and phylogenetic relationships was established among 24 metallothioneins sequences with the bioinformatics ClustalW, MEME and MEGA 4. Finally, it was obtained a common domain for all sequences and close relationship among them, which allowed us to probe that the three sequences under study shared the characteristics of type-3 plant metallothionein."