REDBIO México 2010

Folio: 434
Tipo de presentación: Cartel
Tema: Estrés Biótico

Analysis of differential gene expression of some flavonoids pathway derived genes in Musa spp.-Mycosphaerella fijiensis Morelet pathosystem

Milady Mendoza-Rodríguez1*, Orelvis Portal1, Aminael Sánchez-Rodríguez1, Bárbara Ocaña1, Mayra Acosta-Suárez1, Berkis Roque1, Elio Jiménez1, Monica Höfte2
1Instituto de Biotecnología de las Plantas. Universidad Central ‘Marta Abreu’ de Las Villas. Carretera a Camajuaní km 5.5. Santa Clara, Villa Clara, Cuba. CP 54 830 e-mail: milady@ibp.co.cu 2 Laboratory of Phytopathology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Gent, Belgium

RESUMEN (ABSTRACT)
"Phenylpropanoid pathway is the best-studied, most engineered and frequently induced by pathogens or pathogen elicitors and specifically flavonoid branch (including isoflavonoid), have been subject for the study of different host-pathogen interaction. Differential expression of genes such as chalcone synthase (CHS), flavonoid 3’5' hydroxylase-like (F3’5’H) and isoflavone reductase-like (IRL), involved in flavonoid pathway were investigated in banana plants ‘Calcutta 4’ and ‘Grande naine’ at 0, 6 and 12 days after artificial inoculation with M. fijiensis and after plant elicitation with etephon 500 _M, throughout reverse transcriptase-polymerase chain reaction (RT-PCR) by using specific primers. Gene activation patterns were obtained for three genes under study with a faster and slightly increased in the resistant genotype compared with the susceptible one in the face of fungal challenge. After plant elicitation treatment no significance differences were observed between F3’5’H and IRL mRNA transcripts levels in the susceptible plants however, in the resistant genotype there were observed maximum peaks of expression for each gene under study. The preliminary results in Musa-M. fijiensis pathosystem study constitute the first approach to unravel flavonoids role in plant defense response."