REDBIO México 2010

Folio: 291
Tipo de presentación: Oral
Tema: Micropropagación y Cultivo De Tejidos
Este trabajo se presentará en "Presentaciones Libres 7: Micropropagación, Cultivo de Tejidos y Transformación" el Miercoles dentro del horario 14:20 a 16:20

Development of a Protocol for the Production of Cassava Doubled-Haploids and its Use in Breeding

Zaida Lentini * (1), Changhu Wang (2), Manuel Quintero (3), Hernán Ceballos Corresponding author (3).
* Autor para correspondencia (Corresponding author) zlentini@icesi.edu.co

1- Universidad Icesi, Facultad de Ciencias Naturales. Calle 18 No. 122-135. Cali, Colombia ; 2- South China Botanical Garden,Chinese Academy of Sciences, Xingke Road 723, Guangzhou 510650; 3- CIAT (International Center for Tropical Agriculture). A.A. 6713. Cali, Colombia

RESUMEN (ABSTRACT)
"Cassava is an important crop for subsistence farming in tropical and subtropical regions. Globalization of world economy offers cassava new opportunities for becoming an even more important source of raw materials for industrial uses. Cassava is grown in marginal environments that will likely face serious challenges as a result of climatic changes, requiring a dynamic process to adapt to these environmental conditions. Cassava breeding is cumbersome and inefficient compared to other crops. Doubled haploid plants derived from zygotic haploid cell cultures can be used for practical applications (i.e. breeding, mutagenesis, genetic transformation) or for basic research (functional genomics, gene discovery, proteomics, biochemical, physiological, and developmental biology). Recently, international initiatives recognized the fundamental importance of doubled haploid (DH) science and technology for both plant science research and commercial success in plant breeding. Doubled haploids (inbreeding) in cassava genetic enhancement would create a baseline for development of populations aimed at identification of high-value recessive traits, production of genetic stocks, and application of molecular tools in breeding. Inbred progenitors will make breeding, maintenance, exchange, conservation and exploitation of germplasm much more efficient, increasing the impact of genetic transformation and molecular markers, and genetic stocks based on (self-pollinated) botanical seed easily shared. This work illustrates the development of a reliable system for the production of multi-cellular, pre-embryo structures from isolated microspores of cassava. Next steps involve the expansion of the protocol to other genotypes, the recovery of embryos and their regeneration into plants that can be grown in the greenhouse or the field."