REDBIO México 2010

Folio: 185
Tipo de presentación: Oral
Tema: Micropropagación y Cultivo De Tejidos
Este trabajo se presentará en "Presentaciones Libres 2: Micropropagación, Cultivo de Tejidos y Transformación" el Martes dentro del horario 14:20 a 16:20

"In vitro propagation of Hylocereus purpusii Britton & Rose, a mexican species in danger of extinction"

Daniel Rojas * (1), Manuel de Feria (2), Maité Chávez (2), Mireya Reyna (1), Elisa Quiala (2), Juan Solís (1), Domingo Ruvalcaba (1).
* Autor para correspondencia (Corresponding author) drojas@cuci.udg.mx

1- Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Ocotlán, Jalisco, México; 2- Instituto de Biotecnología de las Plantas, Universidad Central “Marta Abreu” de Las Villas, Carretera a Camajuaní km 5.5, Santa Clara, Villa Clara, Cuba

RESUMEN (ABSTRACT)
"The purpose of this work was to evaluate different conditions and culture parameters for the in vitro establishment and multiplication of Hylocereus purpusii. Seeds were used as vegetal material and a workflow was developed as an alternative for the propagation and recovery of this species in danger of extinction. In the establishment phase, the best result was obtained in the treatment with 1% NaOCl for 15 minutes which resulted in a 90% of germinated seeds and in a final 77.7% of in vitro established plants. In the multiplication phase when combining 8.88 µM of 6-BAP with different concentrations of IAA, a significant increment in the length of the shoots was observed with respect to the control (no addition of IAA). A combination of 8.88 µM of 6-BAP with 3.42 µM of IAA was selected as the best multiplication condition giving a multiplication rate of 8.8. In the acclimatization phase, after two months of culture in ex vitro conditions, the survival was higher than 98% and after six months of growth, the plants were transplanted to field."