REDBIO México 2010

Folio: 114
Tipo de presentación: Oral
Tema: Estrés Biótico
Este trabajo se presentará en "Presentaciones Libres 5: Estrés" el Martes dentro del horario 14:20 a 16:20

Isolation of genes differentially expressed during the defense response of Cassava (Manihot esculenta Crantz) to whitefly (Aleurotrachelus socialis Bondar) attack

Adriana Bohorquez * (1), Bernardo Arias (1), Anthony Bellotti (1), Joe Tohme (1).
* Autor para correspondencia (Corresponding author) abohorquez@cgiar.org

RESUMEN (ABSTRACT)
"Whiteflies can cause major yield losses in cassava in the Americas, Africa and Asia. Of the different whiteflies species Aleurotrachelus socialis is the most damaging whitefly species in northern South America. Systematic evaluation of the cassava germplasm bank (over 5,000 accessions) at CIAT resulted in the identification of cultivars with high levels of resistance to A. socialis. A major source of host resistance in cassava is genotype M Ecu-72. In this work, subtractive libraries and microarrays are used to investigate the defense mechanisms of cassava in response to A. socialis attack, at the gene expression level. M Ecu-72 (resistant) and CMC40 (susceptible) genotypes were infested with A. socialis adults using clip cages, for the isolation of differentially expressed sequences using two strategies: First, the genotype M Ecu-72, infested with A. socialis, was used as tester, while infested CMC40 was used as driver to identify constitutively expressed resistance genes. Second, infested M Ecu-72 was used as tester, while the driver was the same non-infested genotype to obtain genes implicated in the downstream signaling cascade leading to the resistance phenotype. So far, a total of 6 different subtractive cDNA libraries have been generated from leaves. Unique cDNA bands enriched or mainly expressed in the resistant M Ecu-72 line have been isolated and cloned. These cDNAs might represent genes related with the resistance to A. socialis. Microarray-expression profiling was used to identify putative, early response, regulatory, defense-related, and/or signaling genes involved in the resistance of MEcu-72 against A. socialis. Results obtained from the analysis of microarrays will be presented."